WWW. zgys. org 研究抢文 自含式压力蒸汽灭菌生物指示剂抱子计数方法的考察 肖璜l 郭志龙2 夏梦菲l 刘鹏1 马仕洪I 胡昌勤1 (1.中国食品药品检定研究院 北京 l侧50;2. 深圳市药品 检验所) 摘 要 目的:研究自含式压力蒸汽灭菌生物指示剂抱子计数方法。方法:采用活菌计数法，将不同厂家产品在不同洗脱 液、不同平皿计数方式下的回收菌数进行计数，并利用单因素方差分析方法(ANOVA) 对不同处理纽回收茵数的几何均数进行 统计学检验。结果:洗脱液为灭菌纯化水，培养基为膜路陈大豆琼脂培养基，纸片破碎形式为涡旋振荡法(加入攻璃珠) ，平 皿计数形式采用浇碟法的操作方法能获得较好的实验结果。结论:在保证自含式压力蒸汽灭菌生物指示剂的抱子计数菌数 回收率的情况下采用上述操作能降低实验成本且易于操作。 关键词 自含式生物指示剂;压力蒸汽灭菌;11包子计数 中固分类号:R917 文献标识码:A 文章编号:1 ∞8-049X( 2015 )07-1110 -03 Investigation of Spore Counting Methods Using Self-contained Pressure Biological Indicator for Steam Ster- ilization 1 2 1 1 1 Xiao Huang , Guo Zhilong , Xia Mengfei , Liu Peng , Ma Shihong' , Hu Changqin (1. National Institutes for Food and Drug Control , Beijing 1 创)()50 ， China; 2. Shenzhen Institute for Drug Control) ABSTRACT Objective: To study the methods for spore counting using self-contained pressure biological indicator for steam steriliza- tion. Methods: A live bacteria counting method was applied in the recycling bacteria counting using the products from different manu- facturers with various eluent and plant-counting methods , tbe ANOVA analysis was used to statistically detect the di旺erence in the geo- metric average value of the recycling counting among the different treåtment groups. R臼ults: Sterilized purified water as the eluent , soybean-casein digest agar medium as the culture medium , vortex oscillation (with glass beads) as the broken form and pouring plate operation as the plate counting form would obtain beUer experimental results. Conclusion: Under the condition described above , the cost is reduced witb easy operation and promising recoveηof spore counting using self-contained pressure biological indicator for steam sterilization. KEY WOR